BLAST was used to perform a similarity-based search of the GenBank databases. Rohu (Labeo rohita) Geographical Distribution. The total maritime zone of Pakistan is over 30 percent of the land area. Received: 21 April 2020; Accepted: 24 September 2020;Published: 04 November 2020. Ornamental purposes species have been dyed to make them more attractive and sometimes sold to unsuspecting keepers! 3:e422. .woocommerce-account .woocommerce-MyAccount-content { DNA barcoding is a useful tool for the identification of marine fishes from Japan. Identification of freshwater fish species based on single nucleotide polymorphism data analysis. ['_setCustomVar', 1, 'logged-in', 'no', 1], 81] Abstract: Fishing is the most important economic activity in the coastal area of Pakistan, contributing to employment, income generation and export revenues.Marine production has increased more than ten-fold while inland production has increased more than twenty-fold over the last 50 years, largely as a result of government assistance. Introduction to Tilapia Fish Farming:. The digital data collected by the next-generation storage system can also be used to complement the barcode sequences for all fish species. Schneider Riffle Minnow . doi: 10.1080/10408398.2016.1264361, PubMed Abstract | CrossRef Full Text | Google Scholar, Ardura, A., Planes, S., and Garcia-Vazquez, E. (2013). "agent": "-5.5.3-2.2.1" Although its share of the GDP currently is 0.9%, Pakistan earns 6% of its total foreign exchange earnings by exporting fishes, shrimps and other fish products 5. Genetic approach for species identification: forensic application of DNA barcoding. Biochem. img.emoji { The fish has a transparent body which reveals its internal organ hence the name Indian X-ray. Impact Factor 3.661 | CiteScore 4.4More on impact ›, Technological University of Panama, Panama. From the total catch of 589,731 m.tons in 1997, the total marine catch is about 422,201 MT. T have to worry about marketing your products done in the saline of! PLoS One 7:e36586. There are two exotic and 26 indigenous cold water fish species in Pakistan, mainly restricted to the north of the country's North West Frontier Province and Northern Areas. Living species range from the primitive jawless lampreys and hagfishes through the cartilaginous sharks, skates, and rays to the abundant and diverse bony fishes. Pakistan is rich in Sea Fish resources having 814Km coastal line and vast variety of Sea Fish which are exported in significant volumes worldwide. Plant Mol. METRO being a Whole Sale Cash & Carry source Fresh Water Fish directly from the Farms and serves to the HoReCa customers specially Fish Fryers in parallel competition to the Local wet markets but at higher Quality Standards to revolutionized this industry in Pakistan. Oreochromis niloticus belongs to the family Cichlidae and order Cichliformes. Feeding. doi: 10.3897/zookeys.365.6409, Armani, A., Guardone, L., Castigliego, L., D’Amico, P., Messina, A., and Malandra, R. (2015). Sci. Table 1. Comparison of the nucleotide compositions between freshwater and marine fish species. Pakistanis have access to different kinds of fish – those that have been found native in Pakistan, which primarily includes Gourami fish, along with various other fish that can be brought in from the rest of the world for the enthusiastic aquarium owner. "source": "woocommerce", However, Labeo calbasu is distantly related and clustered separately with respect to the other freshwater fish species. "agent": "woocommerce-4.7.1-2.2.0" The use of molecular approaches for identifying fish species has been suggested to mitigate the limitations associated with morphological based identification systems and the lack of local fish identification expertise (Zhang and Hanner, 2011; Keskin and Atar, 2013; Di Pinto et al., 2015). doi: 10.1371/journal.pone.0004379, Keskin, E., and Atar, H. H. (2013). NM helped in English editing. The average interspecific genetic distance among species was 0.311%, compared with 0.308% for genera. The intraspecific diversity of the COI gene in animals had lower resolving power than interspecific diversity as a DNA barcode. Pakistan is proud to have a wide variety of fish species in its seas all across the country. } On the coast of Pakistan, there are more than 30 species of shrimps, 10 species of crabs, 5 species of lobster and about 70 commercial species of fish including sardine, Hilsa, shark, Mackerel, Butterfish, Pomfret, Sole, Tuna, sea bream, Jew fish and Cat Fish, Shark, and Eel. Ich: The Most Common Parasite. }, false ); See All How to Fish Content . Also Ref. Table 5. 58, 1495–1511. (2010). box-shadow: none !important; The raw fish samples obtained were thoroughly washed, immediately transported to the laboratory in polythene bags and stored at –80°C until DNA extraction. [CDATA[ In these page, we also have variety of images available. The 2000-2001 GEP-ISF collaborative exercise on mtDNA: assessing the cause of unsuccessful mtDNA PCR amplification of hair shaft samples. The PCR amplification of Cyt b consisted of the initial denaturation at 95°C for 30 s, followed by 40 cycles of denaturation at 94°C for 30 s, annealing at 50°C for 35 s, extension at 72°C for 120 s, a final extension at 72°C for 4 min, and then an infinite hold at 4°C. doi: 10.1016/j.bse.2010.12.017, Keywords: marine water, DNA barcoding, mislabeling, QR barcodes, fish, freshwater, SNPs, identification, Citation: Ghouri MZ, Ismail M, Javed MA, Khan SH, Munawar N, Umar AB, Nisa M, Aftab SO, Amin S, Khan Z and Ahmad A (2020) Identification of Edible Fish Species of Pakistan Through DNA Barcoding. Also very little investment has been made in this sector. Rafique M. (2007). transition-delay: 0ms; width: 100%; Snappers. The reason is, these species can adjust any temperature and environment as well as the taste of water. J. Forensic Sci. While discussing the production process flow, some facts should be considered regarding the problems faced during operations. Distributed in North West Frontier Province, Punjab, Sindh, and Balochistan, Azad Kashmir (Exotic fish imported for ornamental purposes but feral populations are found in the Indus river up to Tarbela and Khalabat pocket) (Ref. If you are looking for types of fish farming in pakistan you’ve come to the right place. Among the 11 freshwater fish species, the observed nucleotide base composition of all analyzed sequences was 56.0% AT (range: 309–659) and 43.96% GC (range: 207–546) (Table 2). Mitochondrial DNA 22, 55–65. Work in lab of AA has been supported by Higher Education Commission, Islamabad, Pakistan under grant # NRPU-6350. SNP data were used for the development of DNA barcodes for each species using an online QR code generator1. The fish species Tenualosa ilisha and Lactarius lactarius belong to families Clupeidae and Lactaridae and orders Clupeiformes and Perciformes, respectively. Finally, all freshwater and marine fish SNP sequences were used to generate scannable QR codes. Frontier province is found in freshwater rivers across much of South Asia pictures,,... Dijon Mustard Sandwich Spread, Gambusia was brought for mosquito control. 40981, 40982. There are 531 species of Fish in Pakistan. The sum of the tree branch lengths was 2.43; 500 bootstrap replicates with the same units that were used to measure the evolutionary distances were used in the phylogeny test. The fish used in this study were treated and handled according to the standard protocols and Ethics Committee of the University of Agriculture, Faisalabad, Pakistan. Ph.D. thesis, UAAR, 220. The aim of this study is to determine the efficacy of the Cyt b gene for the identification of Pakistan’s freshwater and marine fish species. Some of the native fish species in Pakistan are looked at below. High-quality DNA was used for PCR amplification, as reported by Sevilla et al. Kind of fishes in Pakistan. Pakistanis have access to different kinds of fish – those that have been found native in Pakistan, which primarily includes Gourami fish, along with various other fish that can be brought in from the rest of the world for the enthusiastic aquarium owner. color: #ffdd97; The Cyt b primers produced a single amplification product with a read length of 1,141 bp (Figure 2). Genom. Grayish in color while the belly is whitish overfishing in most of the environment about 193 fish... Rohu is a hardy fish which are exported in significant volumes worldwide you can your. Blue sharks, which can weigh over 200lb, and common skate, again reaching over 200lb in weight. Indian Carp feeds mainly on the water surface or sub-surface. Required fields are marked *. 10, 421–430. Resour. 3. DNA barcoding for identification of fish species in the Taiwan Strait. By many fish farmers in Pakistan for best cooked fish dishes released into the river Neelum Dwarf grows! Annu. A digital barcode hologram is ultimately needed to identify fish species by using a barcode reader swiftly. .woocommerce.widget_shopping_cart .total strong { Belly is whitish the monsoon season the said project are Thaila, Moraka and Rohu maritime of. The dataset Fish-Pak are The flora and fauna of Pakistan suffer from a number of problems. Ecol. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. List Of Fish In Pakistan. In Labeo rohita, Ctenopharyngodon idella, and Hypophthalmichthys molitrix, only one unique site was found, while Channa marulius contained two unique sites, Chitala chitala contained three sites, Labeo gonius contained four sites, Ompok bimaculatus and Oreochromis niloticus contained five sites each, and Mystus cavasius contained seven unique sites based on SNPs. (1993). .ast-header-break-point .ast-mobile-menu-buttons .ast-button-wrap .ast-mobile-menu-buttons-minimal { PAKISTAN - Seafood processors and exporters of All type of Fish, Mud crab, Blue Crab, Top shell, Prawns, Lobsters. The amplified PCR products were visualized and sized on 1% agarose gel. We have 32 images about types of fisheries in pakistan including images, pictures, photos, wallpapers, and more. This PCR-RFLP is an attractive approach in identification of species based on enzymes. var _gaq = _gaq || []; The fishing industry has brought a lot of income to the farmers in particular and the country in general. There are over 25 native cold water fish species and three exotic fish species restricted to the northern area of the country. Reduced production cost per unit of fish produced (<30 to 35 percent). Additionally, sequences with maximum similarity (reference sequences with Accession numbers) from the BLAST search were downloaded from the NCBI database for comparison to the experimental species. Goldfish have been dyed to make them more attractive and sometimes sold to unsuspecting keepers. Various DNA biomarkers have been used for fish identification. In Pakistan, 531 species of fish have been identified, among which 233 are freshwater and the remaining 298 are marine fish species. 173, 1–6. Nicolas, V., Schaeffer, B., Missoup, A. D., Kennis, J., Colyn, M., Denys, C., et al. ['_trackPageview']); The species is omnivorous with different feeding styles for different ages. Each SNP fish sequence was pasted in online site described previously and QR codes were generated, respectively. Front. Raceways also offer a much greater ability to observe the fish. window._wpemojiSettings = {"baseUrl":"https:\/\/\/images\/core\/emoji\/13.0.0\/72x72\/","ext":".png","svgUrl":"https:\/\/\/images\/core\/emoji\/13.0.0\/svg\/","svgExt":".svg","source":{"concatemoji":"https:\/\/\/wp-includes\/js\/wp-emoji-release.min.js?ver=5.5.3"}}; Our approach differs from that of Yang et al. Barcode analysis using the cytochrome-b locus could delineate fish for the identification of mysterious specimens in order to recognize unpredicted diversity between them (Meyer and Paulay, 2005; Kerr et al., 2009). Developments in both fishing methods and fishing operations. //